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bioelektricky vecer - tema prednasky a prosba o pomoc
by Radka Haneckova 05 Jun '14

05 Jun '14

zdravim, rada by som vas pozvala na bioelektricky vecer ktory sa bude konat 12. 6. v brmlabe, blizssie info tu: https://brmlab.cz/event/bioelectric_evening zaroven by som chcela poprosit zaujemcov o ucast, aby vyjadrili svoje preferencie k tematike na ktoru nam Iva Svandova bude prednasat. Pre inspiraciu sem davam sylabus predmetu ktory uci - ak vas nieco z toho velmi zaujma, napiste behom tohto tyzdna. dajte ma do cc, inak mi mail nepride (niesom na brmlab mailing liste!). ak by ste naopak chceli niecim prispiet, budem rada :) hlavne by som potrebovala co najprazdnejsi socroom, aby sa tam dalo pohodlne prednasat, a pokial mozno co najmensi level bordelu vseobecne. obavam sa ze vzhladom na moju obhajobo-prijmackovo-skuskovu situaciu tam nestiham den pred akciou nabehnut a upratat, ako to bezne pred akciami ktore organizujem robiebvam, tak vas tymto prosim o pomoc. tu je slubeny sylabus: MEMBRÁNOVÁ TEORIE BIOELEKTRICKÝCH JEVŮ. Nernstova rovnice, pasivní elektrické vlastnosti membrány nervového a svalového vlákna. Kabelová teorie, elektrotonický potenciálm kapacita membrány, prostorová a časová konstatnta. Rychlost vedení v myelinizovaných a nemyelinizovaných vláknech. Saltatorní vedení. PŘÍSTROJE PRO BIOELEKTRICKÁ MĚŘENÍ. Zesilovače, převodníky, stimulátory, osciloskopy, mikroskopy, záznamová média, počítače, sběr dat a jejich analýza. ELEKTROFYZIOLOGICKÉ MĚŘÍCÍ TECHNIKY. Extracelulární záznamy, intracelulární záznamy, napěťový zámek (voltage clamp), terčíkový zámek (patch clamp), elektrody pro snímaní bioelektrických signálů. OBECNÁ ELEKTROFYZIOLOGIE BUNĚK. Měření bioelektrických jevů v nervových a svalových buňkách, membránové potenciály rostlinných buněk. OBECNÁ ELEKTROFYZIOLOGIE TKÁNÍ. Elektrické potenciály živočišných a rostlinných tkání, elektrické orgány ryb, bioelektrická měření v žabích oocytech a v kuřecím zárodku, elektrické jevy v organismu rostlin a živočichů. ELEKTROFYZIOLOGICKÁ MĚŘENÍ IN VITRO. Periferní nervy, tkáňové kultury neuronů, nervosvalový preparát, mozkové řezy. ELEKTROFYZIOLOGICKÁ MĚŘENÍ IN SITU. Vzruchová aktivita v periferních nervech, kosterních svalech, spinální míše a v mozku. Smyslové orgány a receptorové potenciály ve smyslových orgánech. ELEKTROFYZIOLOGICKÁ MĚŘENÍ IN VIVO. Implantované mozkové elektrody, stereotaxe, měření elektrické aktivity v definované části mozku. Teoretické základy měření bioelektrických potenciálů v prostorovém vodiči (těle), elektroencefalogram, elektrokardiogram, elektromyogram, elektroretinogram. chido -- http://chidori.or.cz "I was twenty-five and too old to be unusual." James Watson, The Double Helix

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Re: [Biolab] visit to project biolab
by Radka Haneckova 05 Jun '14

05 Jun '14

Hi Til, thank you! You are of course welcome to visit us, I will gladly discuss with you about our projects and the whole biohackerspace idea. I speak fluent German (with a bit of an Austrian accent though), so I can accomodate to that aswell if you wish. I don't think the laws in my country are much different from yours actually, eg. a lab has to be certified for certain procedures (such as handling GMOs), but a lot of interesting things that are being done in biohackerspace labs across Europe, and in our lab, are doable within the confines of European laws. In fact, some of the most active biohackers are from Germany - there is a group in Munich for example http://biogarage.de/ and there are several people in Berlin aswell. There is a beautiful documentary by a German journalist on DIYBio: http://www.werwiewas.com/de/projekte/die-gen-koeche/ and he has also written a book on the topic. Unfortunately, we and our lab are currently mid-relocation to new premises, so all of the projects are on hold since we are busy with renovation and moving - thus I am afraid you will catch us a bit "boxed in" :) We are part of a larger place (the prague Brmlab hackerspace) which operates continually and I'm sure we can still meet and talk once you are in Prague. Have a nice day and hope to meet you soon, Radka "chido" Haneckova On Thu, Jun 5, 2014 at 7:52 AM, Til Kreuels <kreuels(a)gmx.de> wrote: > Hi Radka and friends, > > I am a German biologist (specialized in plant physiology, circadian rhythms) > and a soon to be retired biology high school teacher at the same time. As I > am very impressed with your great work at Prague (as seen from a distance) I > am wondering if it was possible to start a similar setup/hacker space here > in Germany. Unfortunately the laws are different here and rather > restrictive. So, some of your projects most likely wouldn't be possible over > here. As I am visiting Prague with a student group between June 23 and June > 26 I would like to ask you, if I could come and meet some of you and/or > visit your lab to see a biohacker space in action and have some discussions > with you. > > Please, let me know what you think. > Looking forward to hearing from you ... > > All the best > > Til (Kreuels) -- http://chidori.or.cz "I was twenty-five and too old to be unusual." James Watson, The Double Helix

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Hello from DIYBioTours!
by VISINONI 20 May '14

20 May '14

Association DIYBioTours(France) contact(a)diybio.fr (0033) 06.33.05.94.52 Hello everyone,we are proud to announce the creation of our association DIYBioTours! Being in a process of development, DIYBioTours needs to connect immediately with other independent community laboratories in Europe. We are therefore considering creating a big European network which allows us to have a direct exchange with other laboratories. We believe that this direct exchange between community laboratories will allow us making significant projects on a European scale, at the same time it is an opportunity where we share our experiences, as well as our material supports. If you know well this kind of project or if you want to join us, we will be really happy to discuss with you and it will be a great pleasure meeting each other in the near future. We look forward to hearing from you, the whole team of DIYBioTours wishes you a nice day! Association DIYBioTours

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Léto, zalévání, postrach!
by Bla�ej Pt��ek 19 May '14

19 May '14

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Brmlab's 3D Logo
by 3D Logos Service 12 May '14

12 May '14

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Patrick McDowell's invitation is awaiting your response
by Patrick McDowell via LinkedIn 05 May '14

05 May '14

Patrick McDowell would like to connect on LinkedIn. How would you like to respond? Accept: https://www.linkedin.com/e/v2?e=25yyu2-hutfypx5-5a&a=preRegInvite&tracking=… View Patrick McDowell's profile: http://www.linkedin.com/e/v2?e=25yyu2-hutfypx5-5a&t=npv&tracking=eml-comm_i… You are receiving Reminder emails for pending invitations. Unsubscribe here: http://www.linkedin.com/e/v2?e=25yyu2-hutfypx5-5a&t=goo&tracking=eml-comm_m… Learn why we included this at the following link: http://www.linkedin.com/e/v2?e=25yyu2-hutfypx5-5a&a=customerServiceUrl&ek=i… © 2014, LinkedIn Corporation. 2029 Stierlin Ct. Mountain View, CA 94043, USA

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Patrick McDowell's invitation is awaiting your response
by Patrick McDowell via LinkedIn 29 Apr '14

29 Apr '14

Patrick McDowell would like to connect on LinkedIn. How would you like to respond? Accept: https://www.linkedin.com/e/v2?e=25yyu2-hukunka4-5z&a=preRegInvite&tracking=… View Patrick McDowell's profile: http://www.linkedin.com/e/v2?e=25yyu2-hukunka4-5z&t=npv&tracking=eml-comm_i… You are receiving Reminder emails for pending invitations. Unsubscribe here: http://www.linkedin.com/e/v2?e=25yyu2-hukunka4-5z&t=goo&tracking=eml-comm_m… Learn why we included this at the following link: http://www.linkedin.com/e/v2?e=25yyu2-hukunka4-5z&a=customerServiceUrl&ek=i… © 2014, LinkedIn Corporation. 2029 Stierlin Ct. Mountain View, CA 94043, USA

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Invitation to connect on LinkedIn
by Patrick McDowell 24 Apr '14

24 Apr '14

LinkedIn ------------ I'd like to add you to my professional network on LinkedIn. - Patrick Patrick McDowell Vice President at Cutting Edge PR Medford, Oregon Area Confirm that you know Patrick McDowell: https://www.linkedin.com/e/25yyu2-hueb9wza-2i/isd/5865145775759056899/gtE38… -- You are receiving Invitation to Connect emails. Click to unsubscribe: http://www.linkedin.com/e/25yyu2-hueb9wza-2i/p43YojbPKAVSGMSknifSNWjArcj/go… (c) 2012 LinkedIn Corporation. 2029 Stierlin Ct, Mountain View, CA 94043, USA.

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skříň na chemikálie - havarie
by Jiří Pinkava 20 Apr '14

20 Apr '14

Ahoj, asi jsem to zapoměl zmíňit tak aspoň dodatečně. Před pár dny došlo k pádu police ve skříni na chemikálie. Jedna z polic to nezvládla a neopravitelně se odporoučela dolů. Nic se nerozbylo ani nerozlilo. Krabice s chemikáliemi které teď není kam dát (bývalá police 4) je vedle skříně. Do doby než bude nová skříň dole bych to asi neřešil? P

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SYBR
by Radka Haneckova 09 Apr '14

09 Apr '14

chtela bych pro potreby biolabu zakoupit barvicku na DNA SYBR safe - krom vyssi efektivity je neskodna a nemutagenni, a hlavne ji lze excitovat na rozdil ot EtBr (ktery je toxicky pomerne dost a hlavne na seznamu T+) obycejnym modrym svetlem o vlnove delce ~470 nm (staci obycejne modre LEDky). Na odfiltrovani modreho svetla lze pouzit oranzove bryle (nebo cokoliv oranzoveho a pruhledneho). Life ji prodava v objemu 400 ul a doporucena davka je redeni 1:10000 v bufru kterym se dela gel, co kdyz se rozpocita na nasi velikost vanicky tak to vyjde tak na 100 davek plus minus. http://www.lifetechnologies.com/cz/en/home/life-science/dna-rna-purificatio… nemam problem objednat a vsechno vybavit, jenom bych rada vedela zda zbyly penize z grantu a jestli je na to muzu pouzit. barvicku bych pak pripadne rada vyuzila na druhem kole elektroforetickeho workshopu (je to jedina vec ktera mi k tomu ted chybi, minule jsme pouzivali EtBr ale nemame zdroj svetla pro excitaci ve spravne vlnove delce. taky bych se rada vyhla praci s nebezpecnou latkou. diky za odpoved! chido -- http://chidori.or.cz "I was twenty-five and too old to be unusual." James Watson, The Double Helix

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